KILSTRUP-NIELSEN CHARLOTTE | Università degli studi dell'Insubria

E-mail

[email protected]

Numero telefonico

0331 339 430

Place and date of birth: November, 1964 (Koege, Denmark)

Nationality: Danish

EDUCATION

December 1996: University Degree in Biochemistry at the University of Copenhagen, Denmark.

Experimental thesis: “Identification of a TATA-box in the hIGF1 gene”.

January 1997-December 1998: Milan, Italy – San Raffaele Scientific Institute - Predoctoral studies.

Janurary 1999- December 2001: Milan, Italy – San Raffaele Scientific Institute – PhD in Cellular and Molecular Biology.

December 2003: Ph.D., Molecular and Cellular Biology. Experimental thesis: “Molecular mechanisms underlying the regulation of PBX1 sub-cellular localization”.

2002-2014: Varese, Italy – University of Insubria, Faculty of Science – Assistant professor in Molecular Biology.

2014-present: Varese, Italy – University of Insurbia, Faculty of Science – Associate professor in Molecular Biology

Charlotte Kilstrup-Nielsen has published more than 30 articles in international peer-reviewed journals and she has refereed several per-reviewed papers. Her research has been financed by several Italian and international grants.

Ricerca

Portale IRInSubria

Pubblicazioni in evidenza

Kilstrup-Nielsen C, Berthelsen J, Blasi F, Mavilio F, and Zappavigna V. (1999) The subcellular localization of PBX1 and EXD proteins depends on nuclear import and export signals and is modulated by association with PREP1 and HTH. Genes & Dev. 13, 946-953.

Fognani C, Kilstrup-Nielsen C, Berthelsen J, Ferretti E, Zappavigna V, and Blasi F. (2002) Characterization of PREP2, a paralog of PREP1, which defines a novel sub-family of the MEINOX TALE homeodomain transcription factors. Nucl Acids Res 30, 2043-2051.

Kilstrup-Nielsen C, Alessio M, Zappavigna V. (2003) PBX1 nuclear export is regulated independently of PBX-MEINOX interaction by PKA phosphorylation of the PBC-B domain. EMBO J. 22, 89-99.

Segalla S, Rinaldi L, Kilstrup-Nielsen C, Badaracco G, Minucci S, Pelicci PG, Landsberger N. (2003) Retinoic acid receptor alpha fusion to PML affects its transcriptional and chromatin-remodeling properties. Mol Cell Biol. 23, 8795-808.

Carro S, Bergo A, Mengoni M, Bachi A, Badaracco G, Kilstrup-Nielsen C, Landsberger N. (2004) A novel protein, Xenopus p20, influences the stability of MeCP2 through direct interaction. J Biol Chem 279, 25623-25631.

Mari F, Azimonti S, Bertani I, Bolognese F, Colombo E, Caselli R, Scala E, Longo I, Grosso S, Pescucci C, Ariani F, Hayek G, Balestri P, Bergo A, Badaracco G, Sapella M, Brocolli V, Renieri A, Kilstrup-Nielsen C, Landsberger N. (2005) CDKL5 belongs to the same molecular pathway of MeCP2 and it is responsible for the early-onset seizure variant of Rett syndrome. Hum Mol Genetics 14, 1935-1946.

Mari F, Kilstrup-Nielsen C, Cambi F, Speciale C, Mencarelli MA, Renieri A. (2005) Genetics and mechanisms of disease in Rett Syndrome. Drug Discovery Today. 2, 419-425.

Bertani I, Rusconi, Bolognese F, Forlani G, Conca B, De Monte L, Landsberger N, Kilstrup-Nielsen C. (2006). Functional consequences of mutations in CDKL5, an X-linked gene involved in infantile spasms and mental retardation. J.Biol.Chem. 281, 32048-32056.

Marchi M, Guarda A, Bergo A, Landsberger N, Kilstrup-Nielsen C, Ratto GM, Costa M. (2007) Spatio-temporal dynamics and localization of MeCP2 and pathological mutants in living cells. Epigenetics. 2, 187-197.

Rusconi L, Salvatoni L, Giudici L, Bertani I, Kilstrup-Nielsen C, Broccoli V, Landsberger N. (2008) CDKL5 expression is modulated during neuronal development and its subcellular distribution is tightly regulated by the C-terminal tail. J Biol Chem. 283, 30101-30111.

Bracaglia G, Conca B, Bergo A, Rusconi L, Zhou Z, Greenberg ME, Landsberger N, Soddu S, Kilstrup-Nielsen C. (2009) Methyl-CpG-binding protein 2 is phosphorylated by homeodomain-interacting protein kinase 2 and contributes to apoptosis. EMBO Rep. 10, 1327-1333.

Ricciardi S, Kilstrup-Nielsen C, Bienvenu T, Jacquette A, Landsberger N, Broccoli V. (2009) CDKL5 influences RNA splicing activity by its association to the nuclear speckle molecular machinery. Hum Mol Genet. 18, 4590-4602.

Forlani G, Giarda E, Ala U, Di Cunto F, Salani M, Tupler R, Kilstrup-Nielsen C, Landsberger N (2010) The MeCP2/YY1 interaction regulates ANT1 expression at 4q35: novel hints for Rett syndrome pathogenesis. Hum Mol Genet. 19, 3114-3123. (equal last author)

Williamson SL, Giudici L, Kilstrup-Nielsen C, Gold W, Pelka GJ, Tam PP, Grimm A, Prodi D, Landsberger N, Christodoulou J. (2012) A novel transcript of cyclin-dependent kinase-like 5 (CDKL5) has an alternative C-terminus and is the predominant transcript in brain. Hum Genet. 131:187-200.

Rusconi L, Kilstrup-Nielsen C, Landsberger N. (2011) Extrasynaptic N-methyl-D-aspartate (NMDA) receptor stimulation induces cytoplasmic translocation of the CDKL5 kinase and its proteasomal degradation. J Biol Chem. 286:36550-36558. (equal last author)

Kilstrup-Nielsen C, Rusconi L, La Montanara P, Ciceri D, Bergo A, Bedogni F, Landsberger N. (2012) What we know and would like to know about CDKL5 and its involvement in epileptic encephalopathy. Neural Plast. 2012;2012:728267.

Ricciardi S, Ungaro F, Hambrock M, Rademacher N, Stefanelli G, Brambilla D, Sessa A, Magagnotti C, Bachi A, Giarda E, Verpelli C, Kilstrup-Nielsen C, Sala C, Kalscheuer VM, Broccoli V. (2012) CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons. Nat Cell Biol. 14:911-923.

Bedogni F, Rossi RL, Galli F, Cobolli Gigli C, Gandaglia A, Kilstrup-Nielsen C, Landsberger N. Rett syndrome and the urge of novel approaches to study MeCP2 functions and mechanisms of action. Neurosci Biobehav Rev. S0149-7634 (14).

Bellini E, Pavesi G, Barbiero I, Bergo A, Chandola C, Nawaz MS, Rusconi L, Stefanelli G, Strollo M, Valente MM, Kilstrup-Nielsen C, Landsberger N. MeCP2 post-translational modifications: a mechanism to functionally organize a disorganized protein? Frontiers Cell Neurosci. 8:236. (Equal last author)

Bergo A, Strollo M, Gai M, Sertic S, Di Cunto F, Kilstrup-Nielsen C, Landsberger N. (2014) MeCP2 localizes at the centrosome and is required for proper mitotic spindle organization. J Biol Chem. 290: 3223-3237. (Equal last author)

La Montanara P, Rusconi L, Locarno A, Forti L, Barbiero I, Chandola C, Kilstrup-Nielsen C, Landsberger. Synaptic synthesis, dephosphorylation and degradation: a novel paradigm for a developmentally regulated NMDA-dependent control of CDKL5 J Biol Chem, 290: 4512-4527. (Equal last author)

Bedogni F, Cobolli Gigli C, Pozzi D, Rossi RL, Scaramuzza L, Rossetti G, Pagani M, Matteoli M, Kilstrup-Nielsen C, Landsberger N. (2015) Defects during Mecp2 null embryonic cortex development precede the onset of overt neurological symptoms. Cerebral Cortex bhv078.

Conti V, Gandaglia A, Galli F, Tirone M, Bellini E, Campana L, Kilstrup-Nielsen C, Rovere-Querini P, Brunelli S, Landsberger N. (2015) MeCP2 affects skeletal muscle growth and morphology through non cell-autonomous mechanisms. Plos One 10:e0130183.

Nawaz MS, Giarda E, Bedogni F, La Montanara P, Ricciardi S, Ciceri D, Landsberger N, Rusconi L, Kilstrup-Nielsen C. (2016) CDKL5 and shootin1 interact and concur in regulating neuronal polarization and migration. Plos One 11(2):eO148634.

Cobolli Gigli C, Scaramuzza L, Gandaglia A, Bellini E, Kilstrup-Nielsen C, Landsberger N, Bedgoni F. MeCP2 related studies benefit from the use of CD1 as genetic background. PlosOne, 11, e0153473.

Hector RD, Dando O, Landsberger N, Kilstrup-Nielsen C, Kind PC, Bailey MES; Cobb SR. Characterisation of CDKL5 Transcript Isoforms in Human and Mouse. Plos One, 11, e0157758.

Stefanelli G, Gandaglia A, Costa M, Cheema M, Dimarino D, Barbiero I, Kilstrup-Nielsen C, Ausió J, Landsberger N. Brain phosphorylation of MeCP2 at serine 164 is developmentally regulated and alters its chromatin association. Sci Rep. 6:28295.